Research article: Introducing Digital Pathology in Fast-Frozen Section by Validating the Whole Slide Imaging Scanner Slideview VS200 Research Slide Scanner

Julie Margaret Williams, Jeppe Noack, Mette Christa Zeuthen, Filis Necip, Camilla C. Qvist

Background: The implementation and usage of digital pathology has undergone a huge development in recent years, However, the area of intraoperative consultation has not yet become digitized or fully investigated. The aim of this study was to explore the possibilities to digitize this area and evaluate the consistency between the diagnoses based on the digital slides versus the traditional microscopic review. The whole slide image scanner Olympus VS200 STL proclaimed that it would be able to scan glass slides with wet mounting glue.

Method: To find the optimal scanning profile a field of four different scan profiles were tested in different contexts. The chosen profile was used to generate 126 digital slides from 60 cases. These slides were assessed on three parameters; compliance between diagnosis of the digital and traditional method, compliance with the visual quality and could the scan time including operating time be completed within 180 seconds per slide.

Results: The overall result showed no deviation between diagnoses made with conventional microscope and the digital slide in 83% of the cases and the average operation time was 92.5 seconds.

Conclusion: Olympus VS200 STL has the potential to become implemented in a clinical pathology department for use in intraoperative diagnostics without affecting workflow, diagnostic accuracy, and demonstrates an acceptable time for review (180 seconds per slide).

Key words: Digital pathology, imaging, microscopy, pathology, whole slide image, fast-frozen section

Int. J. Bio. Lab. Sci 2023(12)1:40-47 【PDF】